![]() ![]() Twenty snake venoms were first separated using reversed-phase liquid chromatography, and a post-column split allowed a small fraction to be analyzed with mass spectrometry, while the larger fraction was collected and dispensed onto 384-well plates. To do so, we used a high-throughput screening approach consisting of a miniaturised plasma coagulation assay in combination with a venom nanofractionation approach. ![]() In this study, we applied a combination of analytical and pharmacological methods to identify snake venom toxins from a wide diversity of snake species that perturb coagulation. However, because snake venoms consist of a mixture of numerous protein and peptide components, high throughput characterizations of specific target bioactives is challenging. The mechanisms of action of coagulopathic snake venom toxins are diverse and can result in both anticoagulant and procoagulant effects. Many snake venoms cause severe coagulopathy that makes victims vulnerable to suffering life-threating haemorrhage. Snakebite is a neglected tropical disease that results in a variety of systemic and local pathologies in envenomed victims and is responsible for around 138,000 deaths every year. However, at high doses, such as those injected in natural bites, intravascular thrombosis due to the action of the prothrombin activator may constitute a potent and very rapid mechanism for killing prey. Toxicity experiments performed in mice suggest that, at low venom doses, neurotoxicity leading to respiratory paralysis represents the predominant mechanism of prey immobilization and death. Antivenoms generated against the venoms of these populations showed a pattern of cross-neutralization, corroborating the immunological kinship of these venoms. ![]() PLA(2)s comprised more than 65% of the venoms of these two populations. In addition, C-type lectin/lectin-like protein and venom natriuretic peptide were identified in the venom of specimens from PNG. Proteins belonging to the following seven protein families were identified in the two venoms: phospholipase A(2) (PLA(2)), Kunitz-type inhibitor, metalloproteinase (SVMP), three-finger toxin (3FTx), serine proteinase, cysteine-rich secretory proteins (CRISP), and coagulation factor V-like protein. “The opportunity to collaborate on bringing Razer’s market-leading, gaming-grade hardware to ORIGIN systems customers is a great way to underscore both of our brands’ commitment to gaming in a real and immediately impactful way.The venom proteomes of populations of the highly venomous taipan snake, Oxyuranus scutellatus, from Australia and Papua New Guinea (PNG), were characterized by reverse-phase HPLC fractionation, followed by analysis of chromatographic fractions by SDS-PAGE, N-terminal sequencing, MALDI-TOF mass fingerprinting, and collision-induced dissociation tandem mass spectrometry of tryptic peptides. “Razer and ORIGIN PC have a shared passion for advancing the state-of-the-art in PC performance to support the advanced needs of gamers,” says Min-Liang Tan, Razer founder, CEO and creative director. ORIGIN PC fans can now complement their existing or next ORIGIN PC system with new ORIGIN PC Edition peripherals to dominate the competition. Fully backed by Razer’s Limited Warranty and ORIGIN PC’s Lifetime 24/7 US Based Support, ORIGIN PC and Razer will have customers covered every step of the way. The ORIGIN PC BlackWidow keyboard and Taipan mouse also feature full support of Razer’s Synapse 2.0 cloud technology by automatically synchronizing user profile settings to a cloud server and allowing complete customization of the keyboard and mouse with a single login. The exclusive black and red ORIGIN PC Edition peripherals not only look epic, they feel epic with every click.” ORIGIN PC and Razer worked closely to develop a unique set of peripherals that didn’t alter Razer’s tried and tested leading products. “Our brands carry a perfect synergy with both of our core values focused on always providing the best gaming experience possible. When looking for a peripherals partner, Razer was the clear choice.” said Kevin Wasielewski ORIGIN PC CEO and co-founder. “ORIGIN PC strives to give our customers the best gaming experience from our award winning integration, to our best in class 24/7 lifetime support. The ORIGIN PC BlackWidow keyboard, Taipan mouse and Goliathus mouse pad puts the power of an ORIGIN PC at a user’s fingertips, providing the tools needed to obliterate the competition. Using Razer’s proven formula for delivering the utmost quality and performance, ORIGIN PC chose the best Razer has to offer for ORIGIN PC Edition peripherals with an exclusive black and red backlit color scheme. ORIGIN PC announced today it has partnered with Razer, the industry leader in gaming peripherals, to bring the ultimate keyboard, mouse and mouse pad to ORIGIN PC.
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